Multiple Mutations
Results
Stability Results
Free Gibbs Energy (ΔΔG) from wild-type:
-1.77 kcal/mol
Decreasing stability
Melting Temperature (ΔTm): -6.2
Mutation Details
Position: 138 (Based on: PDB and Uniprot)
Wild-type: TRP
Mutant: TYR
Chain: A
Structure mutations: 2
Wild-type Environment
RSA: None
SST: None
Phi: None
Psi: None
Depth: None
Experimental Details
Temperature: 298.15
pH: 2.5
Method: Thermal
Measure: CD
Pharmacophore Vector
POS: None
ARO: None
NEG: None
SUL: None
NEU: None
ACC: None
DON: None
Substitution Matrices
BLOSUM 62: 0
PAM 250: 1
Sources
Protein Details
Name: Endolysin
Organism: Enterobacteria phage T4
Lenght:
Weight: None
Literature
REFERENCE: BIOCHIM BIOPHYS ACTA 2, 367-83 (1977)
Pubmed: 911878
DOI: 10.1016/0005-2795(77)90166-0
YEAR: 1977