Single-Point Mutation
Results
Stability Results
Free Gibbs Energy (ΔΔG) from wild-type:
-2.5 kcal/mol
Decreasing stability
Melting Temperature (ΔTm): None
Mutation Details
Position: 49 (Based on: PDB and Uniprot)
Wild-type: GLU
Mutant: GLN
Chain: A
Structure mutations: 0
Wild-type Environment
RSA: 0.0
SST: Strand
Phi: -118.5
Psi: 113.0
Depth: 3.53
Experimental Details
Temperature: 298.95
pH: 7.0
Method: GdnHCl
Measure: CD
Pharmacophore Vector
POS: 0
ARO: 0
NEG: -2
SUL: 0
NEU: 0
ACC: -1
DON: 1
Substitution Matrices
BLOSUM 62: -2
PAM 250: -2
Sources
Protein Details
Name: Tryptophan synthase alpha chain
Organism: Escherichia coli (strain K12)
Lenght:
Weight: None
Literature
REFERENCE: J BIOCHEM 4, 915-21 (1979)
Pubmed: 378988
DOI: 10.1093/oxfordjournals.jbchem.a132423
YEAR: 1979