Single-Point Mutation
Results
Stability Results
Free Gibbs Energy (ΔΔG) from wild-type:
4.6 kcal/mol
Stable
Melting Temperature (ΔTm): None
Mutation Details
Position: 49 (Based on: PDB and Uniprot)
Wild-type: GLU
Mutant: MET
Chain: A
Structure mutations: 0
Wild-type Environment
RSA: 0.0
SST: Strand
Phi: -118.5
Psi: 113.0
Depth: 3.53
Experimental Details
Temperature: 298.95
pH: 7.0
Method: GdnHCl
Measure: CD
Pharmacophore Vector
POS: 0
ARO: 0
NEG: -2
SUL: 1
NEU: -1
ACC: -2
DON: 0
Substitution Matrices
BLOSUM 62: -1
PAM 250: 0
Sources
Protein Details
Name: Tryptophan synthase alpha chain
Organism: Escherichia coli (strain K12)
Lenght:
Weight: None
Literature
REFERENCE: J BIOCHEM 4, 915-21 (1979)
Pubmed: 378988
DOI: 10.1093/oxfordjournals.jbchem.a132423
YEAR: 1979