Alanine Scanning
Predictions for Interface Between Chains E and I
| # | Chain | Wild Type | Position | Mutant | Distance to interface | Predicted ΔΔGAffinity (kcal/mol) | Affinity |
|---|---|---|---|---|---|---|---|
| 1 | I | E | 39 | A | 4.784 | -0.523 | Decreasing |
| 2 | E | L | 126 | A | 3.171 | -1.505 | Decreasing |
| 3 | I | L | 47 | A | 2.909 | -1.037 | Decreasing |
| 4 | I | T | 44 | A | 2.913 | -2.04 | Decreasing |
| 5 | E | G | 154 | A | 3.57 | -0.583 | Decreasing |
| 6 | E | S | 101 | A | 3.417 | -0.712 | Decreasing |
| 7 | E | S | 221 | A | 2.785 | -1.878 | Decreasing |
| 8 | E | N | 218 | A | 2.909 | -0.511 | Decreasing |
| 9 | I | V | 43 | A | 2.936 | -1.533 | Decreasing |
| 10 | E | G | 127 | A | 2.936 | -0.785 | Decreasing |
| 11 | E | G | 131 | A | 4.646 | -0.537 | Decreasing |
| 12 | E | S | 125 | A | 3.757 | -1.791 | Decreasing |
| 13 | E | S | 99 | A | 3.265 | -0.175 | Decreasing |
| 14 | E | T | 220 | A | 3.317 | -1.59 | Decreasing |
| 15 | E | N | 62 | A | 3.012 | -0.797 | Decreasing |
| 16 | E | I | 107 | A | 3.474 | -0.739 | Decreasing |
| 17 | E | G | 219 | A | 3.252 | -1.538 | Decreasing |
| 18 | I | G | 40 | A | 2.586 | -0.415 | Decreasing |
| 19 | E | S | 130 | A | 3.465 | -0.297 | Decreasing |
| 20 | E | G | 128 | A | 3.705 | -0.689 | Decreasing |
| 21 | I | S | 41 | A | 3.705 | -0.519 | Decreasing |
| 22 | I | Y | 35 | A | 3.461 | -0.286 | Decreasing |
| 23 | E | G | 100 | A | 2.913 | -1.143 | Decreasing |
| 24 | E | G | 102 | A | 3.328 | -0.556 | Decreasing |
| 25 | E | M | 222 | A | 4.211 | -1.17 | Decreasing |
| 26 | I | L | 37 | A | 3.781 | -0.413 | Decreasing |
| 27 | I | D | 46 | A | 3.251 | -2.242 | Decreasing |
| 28 | E | L | 217 | A | 3.983 | -0.853 | Decreasing |
| 29 | E | T | 33 | A | 3.806 | -0.71 | Decreasing |
| 30 | E | H | 64 | A | 3.408 | -1.778 | Decreasing |
| 31 | E | G | 63 | A | 4.241 | -0.747 | Decreasing |
| 32 | E | N | 155 | A | 2.72 | -1.129 | Decreasing |
| 33 | I | R | 48 | A | 3.012 | -0.759 | Decreasing |
| 34 | E | F | 189 | A | 3.404 | -1.132 | Decreasing |
| 35 | E | Y | 104 | A | 2.586 | -0.981 | Decreasing |
| 36 | E | S | 103 | A | 4.349 | -0.474 | Decreasing |
| 37 | E | Y | 209 | A | 3.442 | -0.597 | Decreasing |
| 38 | I | P | 42 | A | 3.328 | -1.315 | Decreasing |
| 39 | I | R | 51 | A | 4.917 | -0.745 | Decreasing |
| 40 | E | L | 96 | A | 3.716 | -1.154 | Decreasing |
| 41 | E | D | 32 | A | 4.432 | -1.46 | Decreasing |
| 42 | I | L | 45 | A | 2.72 | -1.895 | Decreasing |
| 43 | I | R | 53 | A | 3.17 | -1.207 | Decreasing |
Bar Chart