1.Submission Page

Users can run three types of prediction.

Single Prediction gives single ∆∆G for given antibody-antigen structure and information about single mutation.

Multiple Prediction allows to predict ∆∆G for multiple mutations with list of mutations.

Also, users are able to conduct Alanine Scanning or Saturation Mutagenesis for antibody-antigen interface residues.

2.Result Page- Single Prediction (1/2)

The result page of Single Prediction is divided into three sections.

The Result section gives predicted ∆∆G, mutation information and antibody information.

The Visualisation part shows inter-/intramolecular interactions of wild-type and mutant structures. Using control panel, users are able to turn on/off each interaction and change the representation of antibody-antigen structure.

3.Result Page- Single Prediction (2/2)

The antibody sequence and its Chothia annotation are shown in the Sequence Viewer.

Users are able to download results and interaction files through the Donwload section.

4.Result Page- Multiple Mutation Prediction (1/2)

The predicted ∆∆G and corresponding information are shown in the Result section.

The antibody-antigen complex can be coloured by average ∆∆G if any of mutations occurs more than once at the same position.

5.Result Page- Multiple Mutation Prediction (2/2)

All the predicted ∆∆G are plotted in the Bar Chart.

The result table and Antibody-Antigen complex which of B-factor is replaced with average ∆∆G are downloadable through the Download section.

6.Result Page- Alanine Scanning

Alanine Scanning gives predicted ∆∆G for antibody-antigen interface residues. Same as the previous mutation list prediction, the result is shown in Result table, Visualisation and Bar Chart sections.

7.Result Page- Saturation Mutagenesis

Apart from the Result and Visualisation sections, Saturation Mutagenesis shows a series of predicted ∆∆G for each of antibody-antigen interface reisdues using Heat Map.