Saturation Mutagenesis
Predictions for Binding Pocket Residues
| # | Chain | Wild Type | Position | Mutant | Predicted ΔΔGAffinity (kcal/mol) | Affinity |
|---|---|---|---|---|---|---|
| 1 | A | ALA | 161 | PRO | -0.86 | Decreasing |
| 2 | A | GLU | 198 | PHE | -1.03 | Decreasing |
| 3 | A | ASN | 218 | THR | 0.19 | Increasing |
| 4 | A | HIS | 207 | TYR | -0.49 | Decreasing |
| 5 | A | ALA | 161 | TYR | 0.73 | Increasing |
| 6 | A | PRO | 217 | TYR | 0.68 | Increasing |
| 7 | A | LEU | 214 | VAL | 0.31 | Increasing |
| 8 | A | VAL | 194 | THR | -0.31 | Decreasing |
| 9 | A | ILE | 159 | TYR | 0.64 | Increasing |
| 10 | A | HIS | 201 | TYR | -0.82 | Decreasing |
| 11 | A | TYR | 216 | PRO | -0.26 | Decreasing |
| 12 | A | LEU | 193 | PRO | 0.11 | Increasing |
| 13 | A | ALA | 163 | ASP | 1.27 | Increasing |
| 14 | A | TYR | 219 | CYS | -0.64 | Decreasing |
| 15 | A | TYR | 219 | ARG | -0.68 | Decreasing |
| 16 | A | TYR | 216 | GLU | 0.26 | Increasing |
| 17 | A | HIS | 162 | PHE | -0.38 | Decreasing |
| 18 | A | VAL | 194 | ALA | -0.22 | Decreasing |
| 19 | A | GLY | 158 | GLN | -0.44 | Decreasing |
| 20 | A | TYR | 219 | THR | -0.56 | Decreasing |
| 21 | A | HIS | 197 | LEU | -1.81 | Decreasing |
| 22 | A | ALA | 163 | PHE | 1.63 | Increasing |
| 23 | A | HIS | 162 | TYR | 2.09 | Increasing |
| 24 | A | GLY | 158 | ALA | 0.03 | Increasing |
| 25 | A | TYR | 216 | GLY | -1.94 | Decreasing |
| 26 | A | GLU | 198 | LEU | -1.44 | Decreasing |
| 27 | A | LEU | 214 | CYS | 0.35 | Increasing |
| 28 | A | LEU | 214 | GLN | -0.45 | Decreasing |
| 29 | A | HIS | 207 | TRP | -0.48 | Decreasing |
| 30 | A | TYR | 219 | MET | -0.03 | Decreasing |
| 31 | A | LEU | 214 | GLU | 0.78 | Increasing |
| 32 | A | HIS | 162 | CYS | 0.2 | Increasing |
| 33 | A | PRO | 217 | HIS | 1.2 | Increasing |
| 34 | A | HIS | 201 | ILE | -1.98 | Decreasing |
| 35 | A | HIS | 197 | ASP | -0.92 | Decreasing |
| 36 | A | PRO | 217 | CYS | 0.28 | Increasing |
| 37 | A | TYR | 216 | ASN | -1.97 | Decreasing |
| 38 | A | ALA | 163 | TYR | 1.38 | Increasing |
| 39 | A | ALA | 161 | THR | 0.33 | Increasing |
| 40 | A | ALA | 161 | GLN | -0.46 | Decreasing |
| 41 | A | ILE | 159 | VAL | 0.58 | Increasing |
| 42 | A | LEU | 214 | GLY | 0.02 | Increasing |
| 43 | A | LEU | 214 | THR | 0.06 | Increasing |
| 44 | A | GLY | 158 | THR | -0.06 | Decreasing |
| 45 | A | HIS | 197 | ASN | -2.18 | Decreasing |
| 46 | A | HIS | 201 | THR | -1.83 | Decreasing |
| 47 | A | HIS | 197 | ILE | -2.4 | Decreasing |
| 48 | A | ILE | 159 | GLU | 0.93 | Increasing |
| 49 | A | LEU | 160 | PRO | -1.67 | Decreasing |
| 50 | A | ILE | 159 | PRO | 0.13 | Increasing |
| 51 | A | LEU | 160 | THR | -0.67 | Decreasing |
| 52 | A | HIS | 207 | GLY | -3.42 | Decreasing |
| 53 | A | TYR | 219 | ILE | -1.06 | Decreasing |
| 54 | A | LEU | 160 | ASP | -0.93 | Decreasing |
| 55 | A | HIS | 162 | GLU | -0.26 | Decreasing |
| 56 | A | ASN | 218 | MET | 0.6 | Increasing |
| 57 | A | LEU | 160 | ILE | -1.3 | Decreasing |
| 58 | A | LEU | 160 | PHE | -1.43 | Decreasing |
| 59 | A | HIS | 197 | SER | -1.6 | Decreasing |
| 60 | A | TYR | 216 | HIS | -1.48 | Decreasing |
| 61 | A | LEU | 193 | ASN | 0.02 | Increasing |
| 62 | A | GLU | 198 | ALA | -1.41 | Decreasing |
| 63 | A | HIS | 201 | ASN | -1.18 | Decreasing |
| 64 | A | HIS | 207 | GLN | -4.01 | Decreasing |
| 65 | A | ALA | 163 | ASN | 0.63 | Increasing |
| 66 | A | HIS | 207 | GLU | -3.53 | Decreasing |
| 67 | A | HIS | 201 | LYS | -1.62 | Decreasing |
| 68 | A | TYR | 216 | LYS | -0.25 | Decreasing |
| 69 | A | TYR | 216 | THR | -1.93 | Decreasing |
| 70 | A | GLU | 198 | SER | -1.34 | Decreasing |
| 71 | A | HIS | 197 | CYS | -1.76 | Decreasing |
| 72 | A | PRO | 217 | PHE | 0.58 | Increasing |
| 73 | A | LEU | 193 | ASP | 0.25 | Increasing |
| 74 | A | HIS | 201 | MET | -1.61 | Decreasing |
| 75 | A | ALA | 163 | GLN | 0.2 | Increasing |
| 76 | A | HIS | 201 | PRO | -1.7 | Decreasing |
| 77 | A | GLU | 198 | ASP | -0.44 | Decreasing |
| 78 | A | GLU | 198 | GLN | -1.68 | Decreasing |
| 79 | A | ALA | 163 | TRP | 1.11 | Increasing |
| 80 | A | LEU | 193 | CYS | 0.31 | Increasing |
| 81 | A | ASN | 218 | GLY | -0.77 | Decreasing |
| 82 | A | PRO | 217 | TRP | 0.51 | Increasing |
| 83 | A | ALA | 161 | HIS | 1.64 | Increasing |
| 84 | A | TYR | 219 | LYS | -0.7 | Decreasing |
| 85 | A | HIS | 207 | SER | -3.52 | Decreasing |
| 86 | A | GLU | 198 | VAL | -1.1 | Decreasing |
| 87 | A | GLY | 158 | SER | 0.14 | Increasing |
| 88 | A | TYR | 219 | GLY | -0.73 | Decreasing |
| 89 | A | GLU | 198 | MET | -1.54 | Decreasing |
| 90 | A | ALA | 163 | PRO | -0.01 | Decreasing |
| 91 | A | TYR | 219 | PHE | -0.28 | Decreasing |
| 92 | A | LEU | 214 | ILE | -0.15 | Decreasing |
| 93 | A | ASN | 218 | ARG | 0.25 | Increasing |
| 94 | A | HIS | 197 | TRP | -1.4 | Decreasing |
| 95 | A | TYR | 216 | ILE | -0.54 | Decreasing |
| 96 | A | LEU | 214 | ASN | -0.01 | Decreasing |
| 97 | A | ALA | 161 | GLY | 0.37 | Increasing |
| 98 | A | ILE | 159 | LEU | 0.2 | Increasing |
| 99 | A | ALA | 161 | ASP | 1.12 | Increasing |
| 100 | A | VAL | 194 | ARG | -0.02 | Decreasing |
| 101 | A | ILE | 159 | ALA | 0.4 | Increasing |
| 102 | A | GLU | 198 | ILE | -1.6 | Decreasing |
| 103 | A | PRO | 217 | ASN | -0.23 | Decreasing |
| 104 | A | ILE | 159 | PHE | 0.97 | Increasing |
| 105 | A | TYR | 216 | ASP | -1.58 | Decreasing |
| 106 | A | LEU | 214 | LYS | 0.54 | Increasing |
| 107 | A | ALA | 163 | LEU | 0.19 | Increasing |
| 108 | A | HIS | 162 | LYS | -0.55 | Decreasing |
| 109 | A | LEU | 193 | HIS | 0.63 | Increasing |
| 110 | A | HIS | 162 | LEU | -0.73 | Decreasing |
| 111 | A | HIS | 207 | ASP | -2.68 | Decreasing |
| 112 | A | GLY | 158 | HIS | 0.08 | Increasing |
| 113 | A | PRO | 217 | SER | 0.28 | Increasing |
| 114 | A | ALA | 163 | THR | -0.05 | Decreasing |
| 115 | A | GLY | 158 | VAL | 0.31 | Increasing |
| 116 | A | TYR | 219 | TRP | -0.34 | Decreasing |
| 117 | A | TYR | 216 | ARG | -1.89 | Decreasing |
| 118 | A | LEU | 193 | TYR | 0.57 | Increasing |
| 119 | A | LEU | 214 | TRP | 0.66 | Increasing |
| 120 | A | TYR | 219 | VAL | -0.62 | Decreasing |
| 121 | A | HIS | 201 | ASP | -0.64 | Decreasing |
| 122 | A | HIS | 162 | VAL | -0.48 | Decreasing |
| 123 | A | GLU | 198 | TRP | -0.28 | Decreasing |
| 124 | A | VAL | 194 | TYR | -0.03 | Decreasing |
| 125 | A | LEU | 214 | PHE | 0.63 | Increasing |
| 126 | A | TYR | 216 | TRP | -1.56 | Decreasing |
| 127 | A | ILE | 159 | CYS | 0.23 | Increasing |
| 128 | A | LEU | 160 | ARG | -0.93 | Decreasing |
| 129 | A | VAL | 194 | ASN | 0.0 | Increasing |
| 130 | A | HIS | 197 | THR | -1.44 | Decreasing |
| 131 | A | TYR | 216 | PHE | -1.57 | Decreasing |
| 132 | A | HIS | 197 | VAL | -1.63 | Decreasing |
| 133 | A | ILE | 159 | ASP | 0.66 | Increasing |
| 134 | A | GLU | 198 | GLY | -1.5 | Decreasing |
| 135 | A | ILE | 159 | THR | 0.0 | Increasing |
| 136 | A | LEU | 160 | MET | -0.66 | Decreasing |
| 137 | A | TYR | 219 | GLU | 0.42 | Increasing |
| 138 | A | HIS | 207 | MET | -2.89 | Decreasing |
| 139 | A | PRO | 217 | ASP | 0.63 | Increasing |
| 140 | A | HIS | 207 | ASN | -3.22 | Decreasing |
| 141 | A | HIS | 201 | ALA | -1.96 | Decreasing |
| 142 | A | LEU | 160 | HIS | -0.25 | Decreasing |
| 143 | A | ILE | 159 | ASN | -0.33 | Decreasing |
| 144 | A | HIS | 201 | LEU | -1.83 | Decreasing |
| 145 | A | HIS | 162 | SER | -0.33 | Decreasing |
| 146 | A | TYR | 216 | ALA | -1.95 | Decreasing |
| 147 | A | ALA | 161 | ARG | 0.27 | Increasing |
| 148 | A | HIS | 162 | PRO | -0.02 | Decreasing |
| 149 | A | PRO | 217 | THR | 0.16 | Increasing |
| 150 | A | VAL | 194 | ILE | -0.72 | Decreasing |
| 151 | A | HIS | 162 | TRP | 0.17 | Increasing |
| 152 | A | ASN | 218 | SER | -0.57 | Decreasing |
| 153 | A | TYR | 219 | GLN | -0.75 | Decreasing |
| 154 | A | ALA | 161 | GLU | 0.67 | Increasing |
| 155 | A | GLY | 158 | GLU | 0.54 | Increasing |
| 156 | A | TYR | 216 | GLN | -2.37 | Decreasing |
| 157 | A | LEU | 160 | SER | -1.04 | Decreasing |
| 158 | A | HIS | 207 | ARG | -3.06 | Decreasing |
| 159 | A | HIS | 207 | CYS | -3.37 | Decreasing |
| 160 | A | TYR | 219 | ALA | -0.47 | Decreasing |
| 161 | A | LEU | 193 | GLN | -0.35 | Decreasing |
| 162 | A | LEU | 214 | PRO | 0.11 | Increasing |
| 163 | A | LEU | 193 | ARG | 0.36 | Increasing |
| 164 | A | LEU | 160 | ALA | -1.71 | Decreasing |
| 165 | A | LEU | 193 | SER | 0.13 | Increasing |
| 166 | A | TYR | 219 | LEU | -0.68 | Decreasing |
| 167 | A | HIS | 197 | ALA | -2.21 | Decreasing |
| 168 | A | GLU | 198 | PRO | -1.31 | Decreasing |
| 169 | A | LEU | 193 | ILE | -0.01 | Decreasing |
| 170 | A | LEU | 193 | VAL | 0.56 | Increasing |
| 171 | A | ASN | 218 | CYS | 0.59 | Increasing |
| 172 | A | PRO | 217 | GLU | 0.94 | Increasing |
| 173 | A | ILE | 159 | HIS | 0.44 | Increasing |
| 174 | A | GLY | 158 | TRP | 0.04 | Increasing |
| 175 | A | ASN | 218 | ASP | 0.52 | Increasing |
| 176 | A | PRO | 217 | MET | 0.23 | Increasing |
| 177 | A | HIS | 207 | LEU | -3.16 | Decreasing |
| 178 | A | GLY | 158 | TYR | 0.06 | Increasing |
| 179 | A | LEU | 214 | ASP | 0.39 | Increasing |
| 180 | A | LEU | 160 | ASN | 0.21 | Increasing |
| 181 | A | TYR | 216 | SER | -1.92 | Decreasing |
| 182 | A | PRO | 217 | ARG | 0.33 | Increasing |
| 183 | A | GLY | 158 | PHE | 0.23 | Increasing |
| 184 | A | HIS | 207 | THR | -3.49 | Decreasing |
| 185 | A | GLU | 198 | HIS | 0.03 | Increasing |
| 186 | A | VAL | 194 | TRP | -0.61 | Decreasing |
| 187 | A | GLY | 158 | ASP | 0.24 | Increasing |
| 188 | A | ILE | 159 | GLY | -0.02 | Decreasing |
| 189 | A | VAL | 194 | MET | -0.74 | Decreasing |
| 190 | A | ALA | 161 | ASN | 0.59 | Increasing |
| 191 | A | VAL | 194 | LEU | -0.99 | Decreasing |
| 192 | A | HIS | 207 | LYS | -3.54 | Decreasing |
| 193 | A | ASN | 218 | VAL | 0.83 | Increasing |
| 194 | A | HIS | 197 | ARG | -1.25 | Decreasing |
| 195 | A | LEU | 193 | MET | 0.22 | Increasing |
| 196 | A | HIS | 197 | PRO | -2.25 | Decreasing |
| 197 | A | ASN | 218 | LYS | -0.81 | Decreasing |
| 198 | A | ILE | 159 | SER | 0.1 | Increasing |
| 199 | A | HIS | 201 | GLN | -2.29 | Decreasing |
| 200 | A | VAL | 194 | ASP | 0.79 | Increasing |
| 201 | A | ALA | 163 | GLU | 1.19 | Increasing |
| 202 | A | HIS | 197 | LYS | -0.73 | Decreasing |
| 203 | A | ALA | 163 | MET | 0.17 | Increasing |
| 204 | A | ASN | 218 | TYR | 0.49 | Increasing |
| 205 | A | ILE | 159 | LYS | 0.11 | Increasing |
| 206 | A | LEU | 160 | GLY | -1.57 | Decreasing |
| 207 | A | TYR | 216 | MET | -0.25 | Decreasing |
| 208 | A | HIS | 162 | THR | 1.52 | Increasing |
| 209 | A | ASN | 218 | TRP | 0.71 | Increasing |
| 210 | A | HIS | 162 | ILE | -1.27 | Decreasing |
| 211 | A | TYR | 216 | CYS | -1.64 | Decreasing |
| 212 | A | HIS | 207 | VAL | -3.12 | Decreasing |
| 213 | A | HIS | 162 | ALA | -0.37 | Decreasing |
| 214 | A | GLY | 158 | CYS | 0.36 | Increasing |
| 215 | A | GLY | 158 | ASN | -0.2 | Decreasing |
| 216 | A | ALA | 161 | TRP | 0.66 | Increasing |
| 217 | A | HIS | 207 | PHE | -0.58 | Decreasing |
| 218 | A | GLU | 198 | THR | -1.61 | Decreasing |
| 219 | A | LEU | 193 | ALA | -0.0 | Increasing |
| 220 | A | LEU | 193 | THR | 0.07 | Increasing |
| 221 | A | VAL | 194 | GLU | 1.09 | Increasing |
| 222 | A | PRO | 217 | VAL | 0.48 | Increasing |
| 223 | A | LEU | 214 | ALA | 0.13 | Increasing |
| 224 | A | ALA | 161 | LYS | 0.07 | Increasing |
| 225 | A | GLY | 158 | ARG | 0.28 | Increasing |
| 226 | A | HIS | 197 | TYR | -0.91 | Decreasing |
| 227 | A | HIS | 201 | TRP | -0.34 | Decreasing |
| 228 | A | TYR | 216 | LEU | -0.31 | Decreasing |
| 229 | A | ALA | 161 | SER | 0.77 | Increasing |
| 230 | A | LEU | 214 | ARG | 0.59 | Increasing |
| 231 | A | LEU | 160 | TRP | -1.0 | Decreasing |
| 232 | A | VAL | 194 | PHE | -0.76 | Decreasing |
| 233 | A | HIS | 197 | GLY | -2.63 | Decreasing |
| 234 | A | LEU | 160 | VAL | -0.86 | Decreasing |
| 235 | A | ASN | 218 | GLN | -0.26 | Decreasing |
| 236 | A | ASN | 218 | LEU | 0.34 | Increasing |
| 237 | A | ALA | 161 | ILE | -0.91 | Decreasing |
| 238 | A | ALA | 163 | LYS | 0.52 | Increasing |
| 239 | A | PRO | 217 | LEU | 0.01 | Increasing |
| 240 | A | HIS | 207 | ILE | -3.65 | Decreasing |
| 241 | A | VAL | 194 | GLN | -0.75 | Decreasing |
| 242 | A | HIS | 197 | PHE | -2.42 | Decreasing |
| 243 | A | PRO | 217 | ILE | 0.05 | Increasing |
| 244 | A | HIS | 162 | ASN | -0.5 | Decreasing |
| 245 | A | VAL | 194 | CYS | -0.16 | Decreasing |
| 246 | A | ASN | 218 | GLU | 0.87 | Increasing |
| 247 | A | VAL | 194 | SER | -0.04 | Decreasing |
| 248 | A | ALA | 161 | CYS | -0.32 | Decreasing |
| 249 | A | ASN | 218 | ILE | 0.28 | Increasing |
| 250 | A | PRO | 217 | GLY | -0.12 | Decreasing |
| 251 | A | TYR | 219 | ASN | -0.25 | Decreasing |
| 252 | A | PRO | 217 | ALA | 0.04 | Increasing |
| 253 | A | ILE | 159 | MET | 0.17 | Increasing |
| 254 | A | GLU | 198 | LYS | -0.98 | Decreasing |
| 255 | A | TYR | 216 | VAL | 0.09 | Increasing |
| 256 | A | HIS | 201 | CYS | -1.49 | Decreasing |
| 257 | A | LEU | 214 | TYR | 2.38 | Increasing |
| 258 | A | GLU | 198 | ASN | -1.3 | Decreasing |
| 259 | A | HIS | 201 | ARG | -1.16 | Decreasing |
| 260 | A | LEU | 193 | GLU | 0.52 | Increasing |
| 261 | A | GLY | 158 | MET | -0.21 | Decreasing |
| 262 | A | LEU | 160 | GLN | -1.36 | Decreasing |
| 263 | A | ASN | 218 | PRO | 1.73 | Increasing |
| 264 | A | HIS | 162 | ASP | -0.38 | Decreasing |
| 265 | A | ASN | 218 | ALA | 0.42 | Increasing |
| 266 | A | LEU | 193 | PHE | 0.42 | Increasing |
| 267 | A | HIS | 201 | GLU | -0.07 | Decreasing |
| 268 | A | VAL | 194 | GLY | -0.22 | Decreasing |
| 269 | A | LEU | 214 | MET | 0.21 | Increasing |
| 270 | A | HIS | 162 | GLY | 2.56 | Increasing |
| 271 | A | HIS | 201 | GLY | -2.14 | Decreasing |
| 272 | A | HIS | 197 | MET | -1.75 | Decreasing |
| 273 | A | HIS | 162 | MET | -0.82 | Decreasing |
| 274 | A | ALA | 163 | GLY | 1.13 | Increasing |
| 275 | A | VAL | 194 | PRO | -0.42 | Decreasing |
| 276 | A | ILE | 159 | ARG | 0.43 | Increasing |
| 277 | A | TYR | 219 | ASP | 0.06 | Increasing |
| 278 | A | ALA | 163 | ARG | 0.25 | Increasing |
| 279 | A | HIS | 197 | GLN | -2.78 | Decreasing |
| 280 | A | HIS | 162 | ARG | -0.37 | Decreasing |
| 281 | A | HIS | 201 | PHE | -2.13 | Decreasing |
| 282 | A | ALA | 161 | VAL | -0.06 | Decreasing |
| 283 | A | ASN | 218 | HIS | 0.83 | Increasing |
| 284 | A | LEU | 193 | LYS | 0.23 | Increasing |
| 285 | A | ALA | 161 | PHE | 0.19 | Increasing |
| 286 | A | LEU | 193 | GLY | 0.01 | Increasing |
| 287 | A | VAL | 194 | LYS | 0.14 | Increasing |
| 288 | A | TYR | 219 | PRO | -0.7 | Decreasing |
| 289 | A | GLY | 158 | LEU | -0.03 | Decreasing |
| 290 | A | HIS | 162 | GLN | 1.26 | Increasing |
| 291 | A | ALA | 163 | CYS | 1.11 | Increasing |
| 292 | A | LEU | 214 | SER | 0.07 | Increasing |
| 293 | A | LEU | 160 | TYR | -1.17 | Decreasing |
| 294 | A | LEU | 193 | TRP | 0.36 | Increasing |
| 295 | A | HIS | 197 | GLU | -0.37 | Decreasing |
| 296 | A | HIS | 201 | SER | -1.67 | Decreasing |
| 297 | A | ALA | 161 | LEU | -0.72 | Decreasing |
| 298 | A | GLY | 158 | PRO | 0.14 | Increasing |
| 299 | A | LEU | 160 | CYS | -1.89 | Decreasing |
| 300 | A | ASN | 218 | PHE | 0.54 | Increasing |
| 301 | A | HIS | 207 | ALA | -3.45 | Decreasing |
| 302 | A | PRO | 217 | GLN | -0.3 | Decreasing |
| 303 | A | GLU | 198 | ARG | -0.33 | Decreasing |
| 304 | A | LEU | 160 | LYS | -0.73 | Decreasing |
| 305 | A | ALA | 163 | SER | 0.35 | Increasing |
| 306 | A | ALA | 163 | ILE | 0.1 | Increasing |
| 307 | A | ILE | 159 | TRP | 1.22 | Increasing |
| 308 | A | PRO | 217 | LYS | 0.05 | Increasing |
| 309 | A | HIS | 201 | VAL | -1.48 | Decreasing |
| 310 | A | VAL | 194 | HIS | 1.12 | Increasing |
| 311 | A | GLY | 158 | ILE | -0.13 | Decreasing |
| 312 | A | GLU | 198 | TYR | -0.45 | Decreasing |
| 313 | A | ALA | 161 | MET | -0.22 | Decreasing |
| 314 | A | HIS | 207 | PRO | -3.44 | Decreasing |
| 315 | A | TYR | 219 | HIS | -0.16 | Decreasing |
| 316 | A | LEU | 214 | HIS | 0.8 | Increasing |
| 317 | A | GLY | 158 | LYS | -0.05 | Decreasing |
| 318 | A | TYR | 219 | SER | -0.51 | Decreasing |
| 319 | A | ALA | 163 | HIS | 2.49 | Increasing |
| 320 | A | ILE | 159 | GLN | -0.1 | Decreasing |
| 321 | A | LEU | 160 | GLU | -0.43 | Decreasing |
| 322 | A | GLU | 198 | CYS | -1.48 | Decreasing |
| 323 | A | ALA | 163 | VAL | 0.72 | Increasing |
Heatmap