StruNHEJ contains a comprehensive catalogue of XRCC4 , DNA PKcs , KU and LIG4 unique variants and holds a diverse range of information, which includes :
- Predicting structural and functional consequences of missense variants in NHEJ using the
mCSM suite of tools.
- Interactive 3D viewer of the crystal structure of the proteins and Lollipop Plots of Variation illustrated within
Pfam domains
- In silico pathogenicity predictions from
PolyPhen-2,
MTR, and
SNAP2, and
PROVEAN.
- Integrated data of variants in all the structures of
the principal components of the NHEJ system- DNA-Lig IV, DNA-PKcs, Ku70/80 and XRCC4 ,in an in silico saturation mutagenesis fashion.
- Novel pathogenicity predictions of missense variants using different
machine-learning classification models trained on features generated by the mCSM platform.
- Interactive plots comparing all variant annotation and metadata variables.
- Interactive 3D viewer for analysing the interatomic interactions of variant
residues within the 3D crystal structure of the proteins
using theArpeggio tool.