DUET predicts the change in protein stability (∆∆G) upon the introduction of a single mutation by combining
two distinct previously published approaches:
To run a prediction:
Your results for a single mutation will be displayed once computations are completed. The results will display the predicted
change in folding free energy upon mutation (ΔΔG in Kcal/mol). A negative value (and red writing) corresponds
to a mutation predicted as destabilising; while a positive sign (and blue writing) corresponds to a mutation
predicted as stabilising. Complementary information also displayed include:
Your results for a systematic prediction will be displayed in a table format with the following information:
In case you experience any trouble using DUET or have any suggestions or comments, please do not hesitate in contacting us (1) either via e-mail or through the online form.