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Pre-calculated example:

The XYLOSE ISOMERASE PROTEIN (RCSB PDB accession 1qt1) binds to a ZN ion. The inputs

  • PDB Chain ID: 1qt1_A
  • Reference Amino Acid: D
  • Alternate Amino Acid: A
  • Position: 244

mutates the aspartic acid at position 244, which is one of the coordinating amino acids. The results indicate reduced potential of binding ZN at this site. See pre-calculated results here.